Coding

Part:BBa_K1983000:Design

Designed by: Vykintas Jaunikis   Group: iGEM16_Vilnius-Lithuania   (2016-08-01)
Revision as of 14:28, 21 October 2016 by Vykintas (Talk | contribs) (→‎Primers)


Codon optimized AvPAL with C-terminal 6XHis-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1265
    Illegal XhoI site found at 1702
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1483
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers

Primers used for amplification of the fragment:

PAL-Chis-ex-FW: AACGTCTCCCATGAAGACCCTGTCCCAG
PAL-Chis-ex-RV: AACTCGAGATGCAGGCTAGGCAGG

Primers used for colony PCR screening:

For pBAD:
Bad-P1: AGATTAGCGGATCCTACCTGACGC
Bad-T1: TGTTTTATCAGACCGCTTCTGCGTTC

For pETDuet-1:
Up-A1: GGATCTCGACGCTCTCCCT
Down3: ACCCCTCAAGACCCGTTTAG

Primers used for cloning into biobrick:

PAL-Chis-BBa-FW: AATCTAGatgaagaccctgtcccagg
PAL-Chis-BBa-RV: AAACTAGTATTAGTGATGGTGGTGGTGATG

Source

E coli

References