Coding

Part:BBa_I746200

Designed by: Narin Hengrung   Group: iGEM07_Cambridge   (2007-08-29)
Revision as of 10:58, 8 September 2007 by Narin (Talk | contribs)

FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.

FepA is an outer membrane receptor protein for Escherichia coli involved in the uptake of the ferric enterobactin siderophore. This part is a deletion mutant of the wild type FepA, which has been shown to convert the protein into an open diffusion channel, similar to a porin. It allows larger molecules through than normally would be permitted. A total of 15 amino acid residues has been deleted from the wild type protein (see Fig. 2A in reference). These have been replaced by a beta turn to aid folding (NCET).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 238
    Illegal AgeI site found at 604
    Illegal AgeI site found at 1231
    Illegal AgeI site found at 1749
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/membrane/channel
Parameters
None