Composite

Part:BBa_K1900002:Design

Designed by: Elise Sloey   Group: iGEM16_WLC-Milwaukee   (2016-10-12)
Revision as of 17:01, 20 October 2016 by Elisesloey (Talk | contribs) (→‎Source)


pBAD+strong RBS+E. coli tolC signal sequence+E. coli tolC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 265
    Illegal NheI site found at 1615
    Illegal NheI site found at 1636
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1555
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 874
    Illegal NgoMIV site found at 1144
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1110


Design Notes

BBa_K206000 was chosen for its ability to be controlled with a common inducer (arabinose) as well as its high rate of transcription. BBa_B0034 was chosen for its high translation efficiency. The main consideration when designing the signaling sequence and E. coli TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible.

Source

BBa_K1406000 comes from the BioBrick registry The tolC signaling sequence and E. coli tolC sequence were synthesized from DNA sequences found in genomic databases

References