Protein_Domain

Part:BBa_K1906004

Designed by: Yuwei Han   Group: iGEM16_XJTLU-CHINA   (2016-09-11)
Revision as of 17:10, 20 October 2016 by Yuwei Han (Talk | contribs)


Elongation Factor Tu

This protein is well known as a translation elongation factor in bacterium Escherichia coli. It can also assemble with phage polymerase domain to form a RNA-dependent-RNA polymerase holoenzyme. It was suggested by XJTLU-CHINA 2016 iGEM team that overexpress of this protein together with EF-TS are essential for the production of Qbeta replicase holoenzyme.

Characterlization=

File:File:20161019150339!SDS-TSTU-REP.jpg
alt text

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 280
    Illegal AgeI site found at 685
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 430


[edit]
Categories
Parameters
None