Protein_Domain

Part:BBa_K1906000

Designed by: Wenbo Xu   Group: iGEM16_XJTLU-CHINA   (2016-07-31)
Revision as of 16:42, 20 October 2016 by Yuwei Han (Talk | contribs)


Elongation Factor Ts

This protein is well known as a translation elongation factor in bacterium Escherichia coli. It can also assemble with phage polymerase domain to form a RNA-dependent-RNA polymerase holoenzyme. It is proved by XJTLU-CHINA iGEM team that overexpress of this protein together with EF-TU are essential for the production of Qbeta replicase holoenzyme.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 688
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 487


[edit]
Categories
//cds/enzyme/endonuclease
//chassis/prokaryote/lactobacillus
Parameters
protein