Project

Part:BBa_K2070005

Designed by: iGEM UT-Tokyo 2016   Group: iGEM16_UT-Tokyo   (2016-10-20)
Revision as of 06:56, 25 October 2016 by RinaSakata (Talk | contribs)


Constitutive ecf35 generator with ecf35 inducible GFP reporter

This part, consisting of a constitutive ECF sigma35 generator BBa_K2070013 and ECF sigma35 inducible GFP reporter BBa_K2070004, was used to test the functionality of ECF sigma35 and its cognate promoter. See experience page for details of the result.


T--UT-Tokyo--BBa K2070005--3.png

Experimental Validation

Measurement of ECF sigma35 and sigma30 promoter activity. Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device BBa_K2070013 and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state.


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