Part:BBa_K2120419
B0015+tetR+B0034+J23109+pTet+B0034+mRFP+B0015
This is one of our application circuit.
This circuit consists of two parts:the inhibitor device and reporter device ,which have already been made into basic parts of biobricks. We choose three different promoters (BBa_J23109,BBa_J23116,BBa_J23106) whose reported strengths are respectively low, medium and strong from constitutive promoter family to express the inhibitor tetR and cI , a reporter protein-mRFP (BBa_E1010) was used as a signal to represent if the concentration of inhibitor controlled by different promoters can repress the expression of downstream gene controllled by inducible promoter completely .At first,we test fluorescence intensity of mRFP protein expressed by each circuit in high copy plasmid pSB1K3 ,the results show that inhibitor devices with J23116 and J23109 cannot inhibit the expression of mRFP ,which means that the strength of these two promoters is too weak to turn off the reporter device in our circuit.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 851
Illegal NheI site found at 874 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1528
Illegal AgeI site found at 1640 - 1000COMPATIBLE WITH RFC[1000]
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