Coding

Part:BBa_K2114020:Design

Designed by: Wladislaw Stroukov   Group: iGEM16_Freiburg   (2016-10-10)
Revision as of 06:37, 20 October 2016 by D.Steinberg (Talk | contribs)

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CgeA_G4S_HA_GST


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1129
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 267
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 526


Design Notes

Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.


Source

The cgeA gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare).