Coding

Part:BBa_K1992001:Design

Designed by: Shilo Ohayon,Asif Gil   Group: iGEM16_Technion_Israel   (2016-10-14)
Revision as of 20:37, 19 October 2016 by Shilo (Talk | contribs) (→‎design notes)

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PctA-Tar hybrid receptor

design notes

The changes in the sequence were 4 nucleotides replacement:
'g' to 'c' - 99 bp
'g' to 'a' - 402 bp
'g' to 'a' - 405 bp
'a' to 'g' - 996 bp


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 270
    Illegal NgoMIV site found at 351
    Illegal NgoMIV site found at 355
    Illegal NgoMIV site found at 374
    Illegal AgeI site found at 474
    Illegal AgeI site found at 685
    Illegal AgeI site found at 1540
  • 1000
    COMPATIBLE WITH RFC[1000]