DNA
Part:BBa_K1976004
Designed by: Thomas Dohmen, Milana Kremenovic, Yannick Kristiansen, Patrick Kunzmann, Tim Maier Group: iGEM16_TU_Darmstadt (2016-10-14)
λ-attP site flanked with two bidirectional terminators
Usage and Biology
λ-Phage attachment-site (attP) for genomic integration flanked by two bidirectional transcription terminators. The Genomic Integration of the λ-phage is catalysed by the λ-Integrase, which recognizes both the bacterial and the viral attachment-site. The viral DNA then is integrated into the bacterial chromosome by recombination.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |