Coding

Part:BBa_K1934090

Designed by: Mathilde Gonthier   Group: iGEM16_INSA-Lyon   (2016-10-13)
Revision as of 17:56, 21 October 2016 by Ylouis (Talk | contribs)


Cellulose Binding Domain 2

CBD2 is a cellulose binding protein present in Part:BBa_K1499004 (streptavidin with CBDs).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Description

Our BLAST analysis revealed 100% identity and an E-value of 3e-63 with a cellulosome anchoring protein of Clostridium cellulovorans. This study enabled us to complete the description of the streptavidin with CBDs part ([[Part:BBa_K1499004]]).

BBa_K1934090 contains the coding sequence of CBD2 as a BioBrick part. Be careful, you will need to put this sequence under the control of a promoter, RBS and terminator in order to use it. Moreover, you can easily combine it with the coding sequence of another protein (eg streptavidin) via a linker to confer a new property: cellulose-binding activity.

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Categories
Parameters
None