Coding

Part:BBa_K2066029:Design

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-07)
Revision as of 03:04, 19 October 2016 by Jlmaniaci (Talk | contribs) (→‎Source)

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pBad - TetR/mCherry Fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1245
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1019
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1001


Design Notes

Codons were optimized for E. Coli expression and non compatible restriction sites were removed.

Source

Linker and mCherry taken from Synthetic Analog Computation in Living Cells (Daniel et al.). TetR from NCBI NC_011812.1

References