Coding

Part:BBa_K2066029:Design

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-07)
Revision as of 03:03, 19 October 2016 by Jlmaniaci (Talk | contribs) (→‎Design Notes)


pBad - TetR/mCherry Fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1245
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1019
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1001


Design Notes

Codons were optimized for E. Coli expression and non compatible restriction sites were removed.

Source

References