Composite

Part:BBa_K1923006

Designed by: Tianyang Mao   Group: iGEM16_Tsinghua   (2016-10-14)
Revision as of 21:37, 18 October 2016 by Maoty (Talk | contribs)


NLS-FLAG-EGFP-dCas9-NLS encoding gene

This composite part is a fusion protein composed of a triple FLAG tag, two SV40 nuclear nuclear localization sequences, a GFP and a dCas9. Sv40 NLS enables the fusion protein to localize in the nuclear. GFP provides fluorescence signal convient for observation under microscope. Protein purification and expression detection can be easily achieved with the help of FLAG tag. dCas9 can be used for CRISPR interference and DNA imaging. We can also utilize dCas9's RNA-binding ability with the help of sgRNA and PAMmer, a NGG-containing DNA oligo.

For more information, please refer to the Wiki page of Team Tsinghua.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1972
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4251
    Illegal XhoI site found at 145
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None