Composite
CXCR5-T2A
Part:BBa_K1993010
Designed by: Su Xiaojun Group: iGEM16_SYSU-MEDICINE (2016-10-13)
Revision as of 15:43, 18 October 2016 by LittleCloud (Talk | contribs)
CXCR5-T2A-Luciferase-IRES-eGFP
Functions:
With the purpose of increasing homing efficiency of MSCs and illustrating their distribution, we constructed a plasmid BBa_K1993010 under the control of EF-1α.
Details:
- Elongation factor-1α (EF-1α), as a constitutive promoter, is responsible for mediating the recruitment of aminoacyl-tRNA to the A-site of the 80S ribosome during protein synthesis.
- CXCR5 is a chemokine receptor and its corresponding ligand is CXCL13. (Details could be seen on BBa_K1993013)
- T2A is a self-cleaving 2A peptide (The average length is 18–22 amino acids) that could be applied in expression of more than one gene in cells. (Details could be seen on BBa_K1993019)
- Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is distinct from a photoprotein. (Details could be seen on BBa_K1993015)
- Internal ribosome entry site (IRES) is an RNA element that allows for translation initiation in an end-independent manner, as part of the greater process of protein synthesis. (Details could be seen on BBa_K1993016)
- eGFP dramatically improved the spectral characteristics of GFP and allowed the practical use of GFPs in mammalian cells. (Details could be seen on BBa_K1993017)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1398
Illegal BamHI site found at 1941
Illegal XhoI site found at 31 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 973
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 259
Illegal SapI site found at 1050
- 10
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Categories
Parameters
None |