![](https://parts.igem.org/images/partbypart/icon_dna.png)
Part:BBa_K2100010:Experience
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2100010
We characterized our hybrid promoter in both MCF-7 and tHESC cells. The following characterization experiment in tHESC cells tested how the hybrid promoter responds to a 1 uM induction of progesterone (MPA). The cells were transfected with 250 ng of hEF1a-mKate as a transfection marker and 250 ng of pHybrid-eYFP for characterization.
The next characterization experiment tested how the hybrid promoter responds to a 10 nM induction of E2 (estradiol) in MCF-7 cells. The cells were transfected with 250 ng of hEF1a-mKate as a transfection marker and 250 ng of pHybrid-eYFP for characterization.
The results showed an 100 fold difference between the yellow fluorescent output of the uninduced tHESC cells and the ones induced with 1 uM MPA.
User Reviews
UNIQ19b3a96603096b18-partinfo-00000000-QINU UNIQ19b3a96603096b18-partinfo-00000001-QINU