Composite

Part:BBa_K1899005

Designed by: iGEM2016_HKUST   Group: iGEM16_Hong_Kong_HKUST   (2016-10-10)
Revision as of 23:34, 17 October 2016 by Leona (Talk | contribs)


J23101-B0032-TetR-B1006- phlFp -GFP

This part is constructed for investigating the effect of tetR over PhlFp.

Results

748px-Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg

Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240(D) and pSB3K3-PhlFp- BBa_E0240(A)

The fold change between pSB3K3-PhlFp - BBa_E0240(A) and negative control (pSB3K3-BBa_E0240) and that of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240(D) is around 13.2 times and 7.01 times respectively.

This is due to the toxicity of BBa_C0040 (tetR). The toxicity reduces the growth rate of the E. coli containing this plasmid. The strong promoter BBa_J23101 makes this effect more significant when doing the characterisation. Though the data are collected with all OD within the mid-log range, there is still a distance between them, making a significant difference in the RFU. It is ungrounded to say tetR interferes the functionality of PhlFp at this stage.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1539


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None