Part:BBa_K1930006

sfGFP(Sp)

sfGFP(Sp) is a reporter gene originally optimized for Streptococcus pneumoniae.

It has been shown when expressed in B. subtilis that the signal is even brighter than the one from sfGFP(Bs). [http://aem.asm.org/content/79/20/6481.full]

Usage and Biology

Images were taken with an Olympus IX71 microscope (Personal DV, Applied Precision; assembled by Imsol, Preston, United Kingdom) using CoolSNAP HQ2 camera (Princeton Instruments, Trenton, NJ, USA) with a 100× phase-contrast objective. Fluorescence filter sets (excitation, 450 to 490 nm; emission, 500 to 550 nm) used to visualize GFP were from Chroma Technology Corporation (Bellows Falls, VT, USA). The exposure times were 0.2 s with 32% excitation xenon light (300 W) for B. subtilis.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]