Signalling

Part:BBa_K1978002

Designed by: Gregor Hoffmann   Group: iGEM16_Goettingen   (2016-10-14)
Revision as of 23:09, 17 October 2016 by To.bi.s (Talk | contribs)


TorA-RFP

The TorA-RFP BioBrick consists of a TorA signal linked to RFP, which is fluorescent protein. The TorA signal sequence allows export of fully-folded proteins through the inner membrane via the TAT(Twin-Arginin Translocation) system. This enables export of RFP out of the cell. The TorA sequence codes for a peptide that harbours a twin-arginine motif. This is vital for the recognition by the Tat system. Moreover, an AxA motif is present, which leads to cleavage by the leader peptidase I (Palmer & Berks 2012). MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATA


Usage and Biology

This BioBrick can be used to test a new organism for compatibility with BioBricks using the standart torA signal sequence from E. coli. -->

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