Part:BBa_K2012001
c-di-GMP tandem riboswitche bc3
Three complete c-di-GMP riboswitches (Bc3, Bc4 and Bc5 RNA) with similar structures, which are arranged in tandem to constitute a triple-tandem (Bc3-5 RNA) riboswitch in the 5′-UTR of the cspABCDE mRNA in Bacillus thuringiensis subsp. chinensis CT-43.
Figure 1. Illustration of riboswitch mechanism.As shown in the illustration, transcript of riboswitches region would form a hairpin and terminate transcription under low concentration of c-di-GMP.
Figure 2. Schematic of riboswitches. (a) Comparison of Bc3, Bc4, Bc5 terminators. Red rectangle shows the GC rich region of three terminators, respectively. (b) Multiply local sequence blast of riboswitches’ terminators. Blue rectangle shows U region of riboswitches.
Figure 3. Quantification of Bc3 riboswitch. We discovered an interesting phenomenon. With no IPTG induction (background concentration of c-di-GMP), the intensity of 73F exceeds 106F, corresponding to 5.2 folds. While under 0.5mM IPTG induction, the terminator forming efficiency of 73F is 39 folds higher than it is without IPTG. Promoter strength of J23106:J23117=1185:162. This indicates that Bc3 riboswitch is responsible for facilitating downstream expression in E.coli, especially under high c-di-GMP concentration.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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