Composite

Part:BBa_K1949101:Design

Designed by: Yoshio Takata   Group: iGEM16_Tokyo_Tech   (2016-10-11)
Revision as of 02:35, 17 October 2016 by Kazuki (Talk | contribs) (→‎Construction)


PBAD-rbs-mazF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

sequence confirmed

Construction

-Strain

All the plasmids were prepared in XL1-Blue strain.

-Plasmids

GFP : Pcon-rbs-gfp (pSB6A1), Plac-rbs (pSB3K3)

MazF : PBAD-rbs-mazF-tt-Pcon-rbs-gfp (pSB6A1) , Plac-rbs (pSB3K3)

References