Coding
SerA

Part:BBa_K2086001

Designed by: Daniel Dooling   Group: iGEM16_UNebraska-Lincoln   (2016-10-13)
Revision as of 21:21, 16 October 2016 by Dannyhdooling (Talk | contribs) (→‎Usage and Biology)


Serine Repeat Antigen (SerA)

The E. coli serA gene encodes the D-3-phosphoglycerate dehydrogenase, which catalyzes the first committed step in the biosynthesis of serine. Serine is an essential amino acid for E. coli growth in minimal medium. Deletion of the serA gene leads to a serine auxotroph, which can be rescued either by the expression of a protein with the same catalytic activity as SerA or by the addition of serine in the growth media.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 293
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Figure 1: The serine biosynthesis pathway.

Serine is an amino acid produced in E. coli K12 through the following metabolic pathway.



[edit]
Categories
//biosafety
//biosafety/semantic_containment
Parameters
None