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Part:BBa_K1936001:Design

Designed by: Marcel Dickmanns   Group: iGEM16_Duesseldorf   (2016-10-13)
Revision as of 21:10, 16 October 2016 by Mdickmanns (Talk | contribs) (→‎References)


Tom5-eGFP-LOV2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 33
    Illegal BamHI site found at 712
    Illegal XhoI site found at 515
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1226


Design Notes

ALERT !!!Under construction !!


Source

Note: All used sequences except for linkers where codon optimized for use in H.sapiens. Therefore base sequences may not match with mentioned sources.


Tom5 yeast sequence:
http://www.ebi.ac.uk/ena/data/view/AY558369

eGFP sequence:

LOV2 sequence:
from pKM291 containing LOVpep[T406A,T407A] double mutant [1]

References

[1] Müller K, Engesser R, Timmer J, Zurbriggen MD, Weber W (2014) Orthogonal optogenetic triple-gene control in Mammalian cells. ACS Synth Biol. 2014 Nov 21;3(11):796-801. doi: 10.1021/sb500305v. Epub 2014 Oct 28.