Translational_Unit

Part:BBa_K2042011

Designed by: Jonathan Tellechea   Group: iGEM16_Evry   (2016-09-02)
Revision as of 21:44, 15 October 2016 by RATOVOMANANA (Talk | contribs)

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RBS +PhaC_resinovorans

This is the sequence of a P.putida ribosom binding site followed by a evolved Pseudomonas resinovorans PHA synthase 1 gene. This translational unit produce a enzyme which take monomer of (D)-lactyl-CoA and produce PLA homopolymer. It was used in the part: BBa_K2042013

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 100
    Illegal XhoI site found at 185
    Illegal XhoI site found at 1043
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 809


[edit]
Categories
//cds/biosynthesis
//chassis/prokaryote/pputida
Parameters
None