Composite

Part:BBa_K2042014:Design

Designed by: Toky RATOVOMANANA   Group: iGEM16_Evry   (2016-10-04)
Revision as of 21:17, 15 October 2016 by RATOVOMANANA (Talk | contribs) (→‎Design Notes)


RBS + PhaC (sp. MBEL 6-19) + RBS + PCT (CP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2386
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
    Illegal NgoMIV site found at 1352
    Illegal AgeI site found at 148
    Illegal AgeI site found at 461
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2792
    Illegal BsaI site found at 3263


Design Notes

We inserted non-coding scar sequences after RBS and close to the gene to prevent missing initial codons during translation. The operon was integrated in pSEVA224 plasmid in order to produce Polylactic acid in Pseudomonas putida.

Source

The RBS were synthesized from the genomic sequence of P.putida, Pct gene were synthetised from genomic sequence of Clostridum propionicum and PhaC gene from Pseudomonas sp. MBEL 6-19

References