Coding

Part:BBa_K2012007

Designed by: Wenqi Huang   Group: iGEM16_HZAU-China   (2016-09-14)
Revision as of 17:03, 15 October 2016 by Johnson Huang (Talk | contribs)


CheZ fussed with a CG linker and reporter GFP(BBa_E0040)

the whole part contains CheZ fused with GFP,which can show the concentration of CheZ. This coding part do not have its RBS and promoter. Users have to add their preferred RBS and promoter by using biobrick assembly or other suitable assembly. We have proved the function of emitting green light under certain exciting light and the function of improving motility by transform it into a cheZ lacking strain, CL1.  
We've also constructed a series of generators with different strength promoter,BBa_K2012013[1], BBa_K2012014[2],BBa_K2012016[3]
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1301


[edit]
Categories
Parameters
None