Generator

Part:BBa_K2012025

Designed by: Jun Li   Group: iGEM16_HZAU-China   (2016-10-07)
Revision as of 14:55, 1 November 2016 by HZAU BGMa (Talk | contribs)

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PcpcG2-172-B0034-sfGFP

sfGFP Generator from pJT119b original plasmid.

 

Figure 1. Fluorescence assay of CcaS-CcaR system with PcpcG2-172 (BBa_K2012015) in CL1 (△EnvZ), and PCB (△CcaS) as chromophore.

Fluorescence under green light is 1.81-folds of red light, proving that green light activates output expression, the device works well. PcpcG2-172 shows high efficiency.

More importantly, leaked expression in darkness significantly reduced after truncation of the constitutive promoter.

 


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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