Generator
Part:BBa_K1934061
Designed by: Mathilde Gonthier Group: iGEM16_INSA-Lyon (2016-10-13)
p66 subunit of HIV reverse transcriptase
This part contains the sequence of the p66 subunit of HIV reverse transcriptase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 238
Illegal BglII site found at 937
Illegal BglII site found at 1100
Illegal BglII site found at 1150
Illegal XhoI site found at 1800 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1739
Illegal AgeI site found at 1067
Illegal AgeI site found at 1182 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 20
Characterization
1. Testing the activity of the part
This part has been transferred into e. coli NM522 for expression. After a 6 hours pre culture phase at 37° , the expression culture was grown overnight at 30°. We the used instructions provided on the NiNTA columns kit from Quigen to purify the protein. We could effectively purify the protein, with a purification yield of 350% and a global yield of 24 %
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Categories
Parameters
None |