Part:BBa_K1951005

FliC E.coli coding sequence

This coding sequence codes the Flagellin C (FliC) protein, filament of the flagellum from Escherichia coli strain.

Flagellin is the main subunit protein which polymerizes to form the filaments of bacterial flagella and is involved to promote bacterial swimming. In the possession of flagellum, bacteria can move what confers them a selective advantage.

It has been demonstrated that Flagellin has the ability to adsorbe precious metal such as Platinum, gold..

Design resum

Codons have been optimized for Escherichia coli allowing a high transcription level.

Every forbidden restriction sites have been removed and sequence is optimital.

Proof of fonctionnality

This sequence has been tested under the control of a strong promoter and validated as fonctionnal. For further information, go on the following link : BBa_K1951008 design

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1224
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 301
  Illegal AgeI site found at 709
  
• 1000
  COMPATIBLE WITH RFC[1000]