Part:BBa_K2012015
PcpcG2-172, a modified PcpcG2 promoter
PcpcG2 promoter is a 238bp green-light activated promoter in Synechocystis PCC 6803(Part:BBa_K592003). The full length promoter is comprised of a G-box region, a CcaR-P activated promoter, and a constitutive promoter, which contributes to the leakiness under red light and low dynamic range. Therefore, we constructed PcpcG2-172, a 172bp truncated cpcG2 promoter deleted for the constitutive promoter.
The mechanism of bio-pattern formation has been studied for nearly half a century, and many theories have been proposed. After entering the 20th century, with the development of Synthetic Biology, more and more biologist start to research it with the ideology of Synthetic, and significant achievements have been achieved. recent years, with the rapid development of computer science, computers have entered almost every household, and became a very important part of works and entertainments in our life. Now, Augmented reality(AR) gradually walk into people's horizons and Man-Computer Interaction bring us new visual stimulation and life feelings. For introducing AR to the study of bio-pattern formation. We use light sense bacterials to make it's movability can be controlled by gene circuits. Then, we created a pattern we wanted in computer and compare it with the shape of the lawn we collect through CCD. The result of compare will be transformed and used to control the move of part bacterial in the colony to change the shape of colony, and it will be the shape in computer finally. Using this technology, we will control the process of bio-tissue development and make organ regeneration precisely possible.
Bio-pattern formation
用synthetic研究bpf
PC
AR
光控制基因回路，然后用计算机比较，然后改菌落形态，得到想要的图案
应用：再生医学
What the Hell!
Have you ever wondered how the stripes on a clownfish come into being? Bio-pattern formation is formation of spatial pattern of a group of cells in morphogenesis. When guided by computer, the spatial pattern of a group of cells can be altered on request, thus establishing bio-pattern formation in Augmented Reality (AR). In our study, a light-switchable E. coli is presented to control synthetic gene circuits including the mobility of cell. By comparing the input pattern with the shape of the lawn in reality collected through CCD, a light induced circuit is generates by the computer, involving regulatory feedback to control the mobility of every bacteria. The morphogenetic fate of each cell is then re-determined, which leads to the transformation of the lawn. Eventually, the shape of the lawn matches the primary input pattern in the computer identically. Utilizing this unparalleled technology, the process of bio-tissue development can be manipulated precisely, not to mention the feasibility of organ regeneration in medical application.
黄师傅
our project is based on the concept of augmented reality.the purpose of our project is to stimulate the enviroment of Ec oli on a Lb PLATE just like the pokemon go.to approve this concept we choose to retrieve its chemotaxis with light.while processing our experiment we find it really hard to concisely regulate the bacteria by light,then we decide to turn to a new tool,the triple-tandem riboswitch to help us concisely augment the reality of bacteria.the application of our idea is beyond imagination,not only chemotaxis,we can also stimulate other enviroments on the surface of a plate like the invasion of phage without interact with real virus which can help improve the safty of biological experiment.ofcourse it also has many other applications.
Terminator！！！
Bio-pattern formation is the establishment of spatial patterns in morphogenesis. When guided by computer, the spatial pattern of a group of cells can be altered in an augmented reality space created by superposing a computer simulated virtual space on a real culture apparatus. In our project, light-switchable synthetic gene circuits are adopted to control the mobility of E. coli cells by light-mediated real-time communication between the real bio-pattern in culture media and its virtual counterpart in computer simulation to implement a synchronized growth. Eventually, the shape of the colony matches the preset pattern we want and thus a “what you see is what you get” platform for the study of bio-pattern formation is obtained. Our system can also be extended to eukaryotic cell community to modulate cell fates in the future which will be particularly useful in aiding the development of biological tissues and the regeneration of organs.
生物模式形成是形态建成中空间格局的建立过程。在计算机的指导作用下，通过在现实的培养皿上叠加一个计算机模拟的虚拟空间，我们可以改变增强现实空间中的细胞群体的空间模式。在我们的项目中，采用光控合成基因回路以控制大肠杆菌细胞的运动性。这里通过现实培养的生物模式与其在计算机模拟中的对应部分之间的光控实时交流，使两者实现同步生长。最终，菌落的形状与我们预设的图案完全吻合，因此建立“所见即所得”的生物模式形成的研究平台。我们的系统在未来可以衍生到真核细胞群落，调节它们的细胞命运对于生物组织发育和器官再生有相当大的帮助。
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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