Coding

Part:BBa_K2110105

Designed by: Haoran Ding   Group: iGEM16_Tianjin   (2016-10-12)
Revision as of 12:19, 15 October 2016 by Hele385738435 (Talk | contribs) (→‎Results)


PETase site-directed mutant I208V

This is one of the site-directed mutant of the PETase gene, we change the 208th amino acid from I to V. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 79
    Illegal BglII site found at 289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Results

We detect the absorption peak of culture medium after culturing with PET film added for 5d. The absorption degree-wavelength curve is as follows. 800px-T--Tianjin--partdataI208V.png


We used the relative fluorescence units to represent the expression level of enzymes. 

                 T--Tianjin--part-I208Vex.png


Finally we got the relative activities of enzymes. 

                 T--Tianjin--part-I208Vbar.png


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Categories
Parameters
chassisSaccharomyces cerevisiae