Part:BBa_K2113005

AMP with glycine

This part contains the anti-microbial peptides (AMP) with consecutive sequences of arginine and tryptophan.These peptides have antimicrobial activity against wide range of microbes. In order to increase the shelf life and stability of the peptide glycine residues are added at the N terminal(GGWRWRWR).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Mobyl@RPBS

3D structural view

ProtParam Results

Number of amino acids: 9
Molecular weight: 1290.51
Theoretical pI: 12.30
Total number of negatively charged residues (Asp + Glu): 0
Total number of positively charged residues (Arg + Lys): 3

Atomic composition:

Carbon (C) 60
Hydrogen (H) 83
Nitrogen (N) 21
Oxygen (O) 10
Sulfur (S) 1

Formula: C₆₀H₈₃N₂₁O₁₀S₁
Total number of atoms: 175

Extinction coefficients:

Extinction coefficients are in units of M^-1 cm^-1, at 280 nm measured in water.

Ext. coefficient: 16500
Abs 0.1% (=1 g/l): 12.786

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Instability index:

The instability index (II) is computed to be 163.60
This classifies the protein as unstable.

Aliphatic index: 0.00

Grand average of hydropathicity (GRAVY): -1.678

References

1. Ressource Parisienne en Bioinformatique Structurale.(Mobyl@RPBS)
2. ExPASy PeptideCutter
3. ExPASy ProtParam