Generator

Part:BBa_K2070016

Designed by: iGEM UT-Tokyo 2016   Group: iGEM16_UT-Tokyo   (2016-10-09)
Revision as of 07:04, 25 October 2016 by RinaSakata (Talk | contribs)


Constitutive ecf16 generator

This part contains an ECF16 coding region downstream of a medium rbs(BBa_B0032) and strong promoter (BBa_J23101). The ecf16 generated can activate the ecf16 promoter (BBa_K2070000).

Usage and Biology

Photograph of plates containing cells transformed with sigma16 related constructs under blue light. From bottom left: BBa_K2070006 (ECF sigma16 inducible GFP reporter), BBa_K2070026 (Constitutive ecf16 generator with ecf 16 inducible GFP reporter), BBa_K2070016 (Constitutive ECF sigma16 generator). As only cells with BBa_K2070026 is shown to fluorescence, it can be suggested that the devices works as expected.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 434
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None