Coding

Part:BBa_I718002:Experience

Designed by: David Puyraimond   Group: iGEM07_Paris   (2007-07-10)
Revision as of 14:29, 6 August 2007 by Davidpz (Talk | contribs) (→‎Applications of BBa_I718002)


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Applications of BBa_I718002

We transformed DGAT gene in pBluescript SK minus vector (Stratagene) (ampicilline resistance and pLac promoter) into E.coli chemically competent (DH5alpha). We used Nile Red fluorescence dye in the medium (5µg/mL medium) to observe lipid inclusions in different conditions (0.4mM IPTG induction in LB medium with or without sodium oleate 2mM).

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UNIQ2547614dcefd6a89-partinfo-00000000-QINU UNIQ2547614dcefd6a89-partinfo-00000001-QINU