![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1976049
Designed by: Alana Gouveia, Franziska Hameister, Jonas Sindlinger, Maik Schork Group: iGEM16_TU_Darmstadt (2016-10-14)
Revision as of 16:59, 23 October 2016 by Jonassindlinger (Talk | contribs)
Colicin E2 immunity protein
Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name .![](https://static.igem.org/mediawiki/parts/9/9b/Minicolicin.png)
Figure 1:Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation. Note: this is not actually this part but BBa_K1976048, a 5 amino acids shorter version
Usage
The Colicin E2 immunity protein (BBa_K1976027 and BBa_K1976028) can be used in combination with the Colicin E2 DNase.
Characteristics
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 46
- 1000COMPATIBLE WITH RFC[1000]
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007
[edit]
Categories
Parameters
None |