Part:BBa_K1923002
Scaffold msr-Scaffold msd-Reverse transcriptase encoding gene
The msr-msd sequence in the part is flanked by two inverted repeats. Once transcribed, the msr-msd RNA folds into a secondary structure guided by the base pairing of the inverted repeats and the msr-msd sequence. The RT enzyme recognizes this secondary structure and uses a conserved guanosine residue in the msr as a priming site to reverse transcripe the msd sequence and produce a hybrid RNA-ssDNA molecule called msDNA. The two BsaI sites enable researchers to design ssDNA sequence produced by this cassette, thus providing a programmable in-vivo ssDNA expression system [2].
For more information, please refer to the Wiki page of Team Tsinghua.
Reference:
[1] Farzadfard F, Lu T K. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations[J]. Science, 2014, 346(6211): 1256272.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 609
Illegal XhoI site found at 363 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 111
Illegal BsaI.rc site found at 95
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