Coding

Part:BBa_K1985000

Designed by: Jasmine Cornish   Group: iGEM16_Kent   (2016-10-13)
Revision as of 10:31, 14 October 2016 by JJECornish (Talk | contribs) (→‎References)


mamP


Usage and Biology

Usage: The mamP gene produces a protein that can be used to transfer electrons to iron molecules. It can be used in combination with other mam genes to form an electron transport complex (reference) that should form magnetic magnetite crystals when the cell is exposed to iron in solution. It was to assemble a construct of MamO, P, T and X in pSB1A3 for in vivo expression.

Biology: MamP is a proposed cytochrome protein (reference) due to the presence of a heme binding motif within its sequence. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.

References

Siponen, Marina I. et al. "Structural Insight Into Magnetochrome-Mediated Magnetite Biomineralization". Nature 502.7473 (2013): 681-684.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 493
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None