Coding
Part:BBa_K2139002
Designed by: UBC iGEM 2016 Group: iGEM16_British_Columbia (2016-10-13)
Coding Sequence of Gluc1C
BBa_K2139002 encompass the coding region for an 1,4-beta-glucosidase known as Gluc1C. Gluc1C is a monomeric enzyme capable of catalyzing the removal of cellobiose from the end of the cellulose polymer. It can be used in conjunction with an exoglucanase for the direct conversion of cellulose into monomeric glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized for use in Caulobacter crescentus and is codon optimized to contain a high GC content.
Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 264
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1138
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Categories
Parameters
None |