Composite

Part:BBa_K2027042

Designed by: Theresa Sievert, Cynthia Hale-Phillips   Group: iGEM16_Stanford-Brown   (2016-10-13)
Revision as of 09:02, 25 October 2016 by Theresasievert (Talk | contribs)


ScoogeOrange-Cellulose Binding Domain-Flag-Lumio-His-tag Fusion Protein

This part was created using a synthetically produced chromoprotein from the DNA2.0 chromoprotein paintbox [1] and adding a cellulose binding domain from the iGEM registry (BBa_K1321366) and a FLAG, lumino and 6x histidine tag.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


References

[1] https://www.dna20.com/products/protein-paintbox#3

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Categories
Parameters
None