Part:BBa_K2116002:Experience
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how you used this part and how it worked out.
Applications of BBa_K2116002
This composite part consists of the ETH promoter NorV (BBa_K2116002), superfolder GFP preceded by one strong ribosomal binding site (BBa_B0034, BBa_K2553008), the NorR regulator (BBa_K4387001), and a double forward (BBa_B0015). We chose a high-copy backbone from Twist for this part. We wanted to compare this ETH NorV promoter to the pNorVβ promoter and see which one was better suited for sensing nitric oxide at lower concentration ranges. According to the data below, the pNorVβ had higher responses to DETA/NO induction than the NorV promoter of the ETH 2016 team when tested at different concentration levels.
This construct was tested in the bacterial strain E.coli Nissle 1917.
Characterization
Measurements
References
Xiaoyu J. Chen, Baojun Wang, Ian P. Thompson, and Wei E. Huang et al. Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells ACS Synthetic Biology 2021 10 (10), 2566-2578 DOI: 10.1021/acssynbio.1c00223
User Reviews
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