Reporter
GG95

Part:BBa_K2145000

Designed by: Laura Xinyu Chen, Melody Wu, Katie Maruna   Group: iGEM16_Alverno_CA   (2016-10-13)
Revision as of 22:41, 30 October 2016 by Sclamons (Talk | contribs)


GFP/RFP reporter (Forward/Forward) with random 500bp spacer

Reporter plasmid to demonstrate the effect of a random 500 bp spacer on reporter expression. This part contains two fluorescent protein coding sites (for RFP and GFP), both facing forward, and was assembled using Golden Gate Assembly. To make the plasmid, parts were amplified from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2226
    Illegal AgeI site found at 2338
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


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Categories
Parameters
None