RNA

Part:BBa_K2020042:Design

Designed by: Andrea Hoeltken, Vroni Czotscher, Carolina Bonerath   Group: iGEM16_Aachen   (2016-10-02)
Revision as of 10:30, 16 October 2016 by Andrea hoeltken (Talk | contribs) (→‎References)


Mj-tRNA with amber anticodon for incorporating ncAA in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

tRNA has 77 amino acids. The ncAA will be attached to the 3' end. On the contrary to aminoacylation with natural amino acids, the acceptor minihelix alone is a substrate for aminoacylation with ncAA's.

Source

wild type tyrosyl Methanococcus janaschii tRNA:Synthetase-pair with anticodon changed to Amber Anticodon CTA. Obtained via iGEM Team 2014 Austin, Texas.

References

  • Zhang et al, 2002, Structure-based design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase for incorporation of O-methyl-L-tyrosine
  • Kobayashi et al, 2003, Structural basis for the orthogonal tRNA specificities of tyrosyl-tRNA synthetasees for genetic code expansion