RNA

Part:BBa_K2020042:Experience

Designed by: Andrea Hoeltken, Vroni Czotscher, Carolina Bonerath   Group: iGEM16_Aachen   (2016-10-02)
Revision as of 21:26, 13 October 2016 by Andrea hoeltken (Talk | contribs) (→‎User Reviews)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2020042

[http://2014.igem.org/Team:Austin_Texas/kit iGEM Team Austin, Texas 2016 - Expanded Genetic Code Measurement Kit]

The tRNA is used together with a tRNA-Synthetase. It has been proven to work with (enter links for parts)

  • Y-RS
  • oNBY-RS
  • AzF
  • CN-F synthetase
  • Iodo-Y synthetase
  • 5HT-P synthetase
  • Nitro-Y synthetase
  • Amino-Y synthetase

[http://2014.igem.org/Team:Austin_Texas/photocage iGEM Team Austin,, Texas - Project: Photocaged T7 RNA Polymerase]

iGEM Team Austin, Texas 2014 insorporated oNBY into T7 RNA polymerase.

[http://2016.igem.org/Team:Aachen/Description iGEM-Team Aachen 2016 - LIPS Light inducible proteases]

iGEM Team Aachen 2016 used the tRNA to successfully incorporate tyrosine, oNB-Y and in E.coli BL21 DE3 gold. Furthermore they used the tRNA with the pFRY reporter for screening their newly designed DMNBS-RS.

User Reviews

UNIQ514fcc53289b11b3-partinfo-00000000-QINU UNIQ514fcc53289b11b3-partinfo-00000001-QINU