RNA

Part:BBa_K2020042:Experience

Designed by: Andrea Hoeltken, Vroni Czotscher, Carolina Bonerath   Group: iGEM16_Aachen   (2016-10-02)
Revision as of 21:23, 13 October 2016 by Andrea hoeltken (Talk | contribs) (→‎iGEM Team Austin,, Texas - Project: Photocaged T7 RNA Polymerase)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2020042

[http://2014.igem.org/Team:Austin_Texas/kit iGEM Team Austin, Texas 2016 - Expanded Genetic Code Measurement Kit]

The tRNA is used together with a tRNA-Synthetase. It has been proven to work with (enter links for parts)

  • Y-RS
  • oNBY-RS
  • AzF
  • CN-F synthetase
  • Iodo-Y synthetase
  • 5HT-P synthetase
  • Nitro-Y synthetase
  • Amino-Y synthetase

[http://2014.igem.org/Team:Austin_Texas/photocage iGEM Team Austin,, Texas - Project: Photocaged T7 RNA Polymerase]

iGEM Team Austin, Texas 2014 insorporated oNBY into T7 RNA polymerase.

[http://2016.igem.org/Team:Aachen iGEM-Team Aachen 2016]

  • [http://2016.igem.org/Team:Aachen/Description LIPS - Light inducible proteases]

iGEM Team Aachen 2016 used the tRNA to successfully incorporate tyrosine, oNB-Y and DMNB-S in E.coli BL21 DE3 gold with their newly designed DMNBS-RS

User Reviews

UNIQ076bd9092d8ebafa-partinfo-00000000-QINU UNIQ076bd9092d8ebafa-partinfo-00000001-QINU

Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a low copy plasmid (e.g. pACYC) which fits your needs.

[http://2016.igem.org/Team:Aachen Team Aachen 2016] used tRNA in pACYC with p15A-ORI, Ipp-Promoter, Terminator, Gent-Resistance. Together with an ncAA-Synthetase (For example: BBa_K2020050) with own promoter and Terminator.