RNA

Part:BBa_K1941001

Designed by: Dimitri Coukos   Group: iGEM16_EPFL   (2016-10-13)
Revision as of 21:10, 18 October 2016 by Alixfailletaz (Talk | contribs)


scTef1_PP7

Scaffold RNA (scRNA) with PP7 hairpin that targets TEF1 promoter.

Usage and Biology

A scaffold RNA is a guide RNA (gRNA), supplemented with an additional hairpin that can recruit different RNA binding module. In the case of this BioBrick, the hairpin is PP7, a well-characterized viral RNA sequence which is recognized by PCP RNA binding module. This allows the recruitment of fusion proteins, like BBa_K1941000, to act on the level of expression of the promoter TEF1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None