Intermediate
Part:BBa_I759002:Design
Designed by: Josh Michener Group: iGEM07_Caltech (2007-06-27)
pTet-spacer-cro
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 174
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A pTet promoter (can be repressed by tet Repressor, and transcription activity can be restored by addition of anhydrotetracycline (aTc)) produces transcripts of a spacer (including the RBS)-cro complex. The spacer is a temporary placeholder, where later cis sequences (for cis riboregulator) will be cloned in.
Source
Caltech iGEM