Composite

Part:BBa_K1976025

Designed by: Patrick Kunzmann   Group: iGEM16_TU_Darmstadt   (2016-10-13)
Revision as of 14:40, 22 October 2016 by Padix (Talk | contribs)


Orthogonal pair for amber supression with OMT

Orthogonal pair for amber supression with OMT

An orthogonal pair for O‑methyl‑l‑tyrosine incorporation at the position of the amber stop codon. The pair ist based on the pairs from the "Expanded Genetic Code Measurement Kit" by the iGEM team Austin Texas 2014. The open reading frame (ORF) of the aminoacyl tRNA synthetase was exchanged with the ORF of the OMT tRNA synthtase BBa_K1976020

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1153
    Illegal BamHI site found at 1159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1185
    Illegal NgoMIV site found at 1645
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None