Composite

Part:BBa_K1937003:Experience

Designed by: Oumnia KARIM   Group: iGEM16_Toulouse_France   (2016-10-13)
Revision as of 12:40, 13 October 2016 by Oumniak (Talk | contribs) (→‎Applications of BBa_K1937003)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1937003

The part was sub-cloned in thepSBBsOK-mini plasmid (BBa_K1937001) for validation (resulting in part BBa_K1937004).In presence of N-acetyl glucosamine in the media, the clones are expected to be red, and white without this substrate Nag.

BBa K1937003-results.png

We indeed observed some red stains on the colonies only with the pNagA promoter growing on NAG (as well as with the pNagP promoter, see part BBa_K1937006), but the expression is late during the colonies development and does not seem to be specific (same result with the control plate on glucose)

User Reviews

UNIQ60c98afb076182be-partinfo-00000000-QINU UNIQ60c98afb076182be-partinfo-00000001-QINU