Part:BBa_K1993032
CXCR4-T2A-Luciferase
With the purpose of increasing homing efficiency of MSCs and illustrating their distribution, we had constructed a composite part that containing coding sequences of chemokine receptor CXCR4 and bioluminescence Luciferase. It’s also convenient for us to construct our plasmids through Gateway technology.
CXCL12 is one of the most common chemokines in various inflammatory diseases, especially in inflammatory bowel disease(IBD). CXCR4, its corresponding chemokine receptor, is responsible for directing T lymphocytes to the lesions. (Details could be seen on BBa_K1993013).
Luciferase is a kind of oxidative enzymes that produce bioluminescence which enable us observing cells in vivo. (Details could be seen on BBa_K1993015).
T2A is a self-cleaving 2A peptide (The average length is 18–22 amino acids) that could be applied in expression of more than one gene in cells. (Details could be seen on BBa_K1993019)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1350
Illegal BamHI site found at 494
Illegal BamHI site found at 1893 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |