Coding

Part:BBa_K1998013:Design

Designed by: Shauna Winchester   Group: iGEM16_Macquarie_Australia   (2016-10-13)
Revision as of 21:59, 18 October 2016 by SWinchester (Talk | contribs)

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MgPPIX to Chlorophyll a plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 912
    Illegal BglII site found at 2365
    Illegal BglII site found at 5647
    Illegal BglII site found at 6136
    Illegal BamHI site found at 316
    Illegal BamHI site found at 3128
    Illegal BamHI site found at 5416
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1904
    Illegal AgeI site found at 1955
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 478
    Illegal BsaI.rc site found at 4018


Design Notes

Codon optimisation for bacteria, order of parts in operon, illegal enzyme kinetics



Source

The genes were sourced from Chlamydomonas rienhardtii and synthesised.

References